제 품 소 개

진단이 바뀌면 미래가 바뀐다.

Sager DNA SEQ

Dye-Terminator Cycle Sequencing

  • Dye-Terminator chemistry utilizes dye-labeled dideoxynucleotides, Which is a nucleotide that is missing the hydroxy groups on both the 2'& 3' carbon atoms of the sugar backbone rendering it incapable of covalently linking ti the next nucleotide in a growing molecule of DNA

유전자분석 서비스

서비스명 구분 세부사항
Single read sequencing Plasmid DNA or PCR product DNA Plasmid : 100ng (DNA 권장농도, Universal 프라이머 표기)
PCR product : 50ng(분석프라이머 10pmol 동봉)
Lamda/ Cosmid or Fosmid/ BAC DNA DNA : 1ug (DNA 권장농도, Universal 프라이머 표기)
Difficult sequencing Nucleotide repeat, Homopolymer or GC-rich 자세한 사항은 당사에 문의
Full length sequencing Primer walking method 프라이머 디자인, 합성비용 포함 Contig 작성 제공 (10kb 미만 권장)
Shot-gun method 5X 기준, Contig 작성 제공 (10kb 이상 권장)
Preparation and purification DNA prep, PCR purification or Gel elution DNA prep : 항생제, Vector 종류 표기
PCR purification : 최소 volume 25㎕(50ng/㎕)
Gel elution : Major band 또는 Target band
PCR reaction Normal/ RT PCR condition, 프라이머 합성 비용 별도

Genotyping analysis

Microsatellite Fragment Sizing

  • Alleles distinguished by the number of repeat units which change the length of the PCR product

What is Genotyping?

  1. To compare genetic composition of different samples to see if they differ in primary structure or sequence
  2. To determine exactly which variant of a known polymorphism is present in a sample